SIMS MICROSCOPY IN THE BIOMEDICAL FIELD

被引:41
作者
FRAGU, P
BRIANCON, C
FOURRE, C
CLERC, J
CASIRAGHI, O
JEUSSET, J
OMRI, F
HALPERN, S
机构
[1] Inserm U66 Équipe de Microscopie Ionique, Relation imagerie chimique quantitative-fonctions cellulaires, Institut Gustave-Roussy
关键词
SIMS MICROSCOPY; HUMAN PATHOLOGY; THYROID; METABOLIC RADIOTHERAPY; CHEMOTHERAPY;
D O I
10.1016/0248-4900(92)90004-K
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We attempted to indicate the requirements for biomedical applications of SIMS microscopy. Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue. Furthermore, it is often necessary to correlate ionic and light microscope images. This implies a common methodological approach to sample preparation for both microscopes. The use of low or high mass resolution depends on the elements studied and their concentrations. To improve the acquisition and processing of images, digital imaging systems have to be designed and require both ionic and optical image superimposition. However, the images do not accurately reflect element concentration; a relative quantitative approach is possible by measuring secondary ion beam intensity. Using an internal reference element (carbon) and standard curves the results are expressed in mu-g/mg of tissue. Despite their limited lateral resolution (0.5-mu-m) the actual SIMS microscopes are very suitable for the resolutio of biomedical problems posed by action modes and drug localization in human pathology. SIMS microscopy should provide a new tool for metabolic radiotherapy by facilitating dose evaluation. The advent of high lateral resolution SIMS imaging (< 0.1-mu-m) should open up new fields in biomedical investigation.
引用
收藏
页码:5 / 18
页数:14
相关论文
共 60 条
[1]   INTRACELLULAR-LOCALIZATION OF DRUGS IN CULTURED TUMOR-CELLS BY ION MICROSCOPY AND IMAGE-PROCESSING [J].
BERRY, JP ;
LESPINATS, G ;
ESCAIG, F ;
BOUMATI, P ;
TLOUZEAU, S ;
CAVELLIER, JF .
HISTOCHEMISTRY, 1990, 93 (04) :397-400
[2]   CHANGES IN I-127 MICE THYROID-FOLLICLE STUDIED BY ANALYTICAL ION MICROSCOPY - A KEY FOR THE COMPREHENSION OF AMIODARONE-INDUCED THYROID-DISEASES [J].
BRIANCON, C ;
HALPERN, S ;
TELENCZAK, P ;
FRAGU, P .
ENDOCRINOLOGY, 1990, 127 (03) :1502-1509
[3]  
BRIANCON C, 1992, IN PRESS J BIOL TRAC
[4]   A MICROCOMPUTER BASED DIGITAL IMAGING-SYSTEM FOR ION MICROANALYSIS [J].
BRYAN, SR ;
WOODWARD, WS ;
GRIFFIS, DP ;
LINTON, RW .
JOURNAL OF MICROSCOPY-OXFORD, 1985, 138 (APR) :15-28
[5]   QUANTITATIVE MICROLOCALIZATION OF DIFFUSIBLE IONS IN NORMAL AND GALACTOSE CATARACTOUS RAT LENS BY SECONDARY ION MASS-SPECTROMETRY [J].
BURNS, MS ;
FILE, DM .
JOURNAL OF MICROSCOPY-OXFORD, 1986, 144 :157-182
[6]   BIOLOGICAL MICROANALYSIS BY SECONDARY ION MASS-SPECTROMETRY - STATUS AND PROSPECTS [J].
BURNS, MS .
ULTRAMICROSCOPY, 1988, 24 (2-3) :269-281
[7]   POLYATOMIC INTERFERENCES IN HIGH-RESOLUTION SECONDARY ION MASS-SPECTRA OF BIOLOGICAL TISSUES [J].
BURNS, MS .
ANALYTICAL CHEMISTRY, 1981, 53 (13) :2149-2152
[8]  
BURNS MS, 1986, SCANNING ELECTRON MI, V4, P1277
[9]  
Castaing R., 1962, J MICROSC-OXFORD, V1, P395
[10]   PROCESSING OF SECONDARY ION-MICROSCOPE IMAGES - AN EXAMPLE OF APPLICATION TO THE THYROID [J].
CAVELLIER, JF ;
BERRY, JP ;
ESCAIG, F ;
BOUMATI, P ;
GAUME, P .
JOURNAL OF MICROSCOPY-OXFORD, 1989, 154 :31-38