IS1136, AN INSERTION ELEMENT IN THE ERYTHROMYCIN GENE-CLUSTER OF SACCHAROPOLYSPORA-ERYTHRAEA

被引：22

作者：

DONADIO, S ^{[1
]}

STAVER, MJ ^{[1
]}

机构：

[1] ABBOTT LABS,CORP MOLEC BIOL,ABBOTT PK,IL 60064

来源：

GENE | 1993年 / 126卷 / 01期

关键词：

MACROLIDE ANTIBIOTIC; POLYKETIDE SYNTHASE; STREPTOMYCES;

D O I：

10.1016/0378-1119(93)90604-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Saccharopolyspora erythraea ervAI and eryAII genes, which, together with eryAIII, are responsible for the formation of the macrolactone portion of the antibiotic erythromycin, are separated by a 1.46-kb segment, designated IS1136, with the characteristics of an insertion sequence. It contains an open reading frame of 425 codons similar to that of the Anabaena IS891 and is present in four nonidentical copies in the Sac. erythraea genome. Inverted repeats were found near the ends of IS1136, and in the copy in eryA, one of the ends was found to overlap the 5' end of eryAII. Hybridization analysis suggests that IS1136 is confined to Saccharopolyspora species containing eryA-homologous DNA.

引用

页码：147 / 151

页数：5

共 18 条

[1] CHARACTERIZATION OF AN INSERTION-SEQUENCE (IS891) OF NOVEL STRUCTURE FROM THE CYANOBACTERIUM ANABAENA SP STRAIN M-131
BANCROFT, I
WOLK, CP
[J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (11) : 5949 - 5954
[2] IDENTIFICATION OF DEBS-1, DEBS-2 AND DEBS-3, THE MULTIENZYME POLYPEPTIDES OF THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE FROM SACCHAROPOLYSPORA-ERYTHRAEA
CAFFREY, P
BEVITT, DJ
STAUNTON, J
LEADLAY, PF
[J]. FEBS LETTERS, 1992, 304 (2-3) : 225 - 228
[3] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX
DEVEREUX, J
HAEBERLI, P
SMITHIES, O
[J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (01) : 387 - 395
[4] DISRUPTION OF A RHODANESELIKE GENE RESULTS IN CYSTEINE AUXOTROPHY IN SACCHAROPOLYSPORA-ERYTHRAEA
DONADIO, S
SHAFIEE, A
HUTCHINSON, CR
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (01) : 350 - 360
[5] MODULAR ORGANIZATION OF GENES REQUIRED FOR COMPLEX POLYKETIDE BIOSYNTHESIS
DONADIO, S
STAVER, MJ
MCALPINE, JB
SWANSON, SJ
KATZ, L
[J]. SCIENCE, 1991, 252 (5006) : 675 - 679
[6] BIOSYNTHESIS OF THE ERYTHROMYCIN MACROLACTONE AND A RATIONAL APPROACH FOR PRODUCING HYBRID MACROLIDES
DONADIO, S
STAVER, MJ
MCALPINE, JB
SWANSON, SJ
KATZ, L
[J]. GENE, 1992, 115 (1-2) : 97 - 103
[7] Galas DJ, 1989, MOBILE DNA, P109
[8] IDENTIFICATION, GENETIC-ANALYSIS AND DNA-SEQUENCE OF A 7.8-KB VIRULENCE REGION OF THE SALMONELLA-TYPHIMURIUM VIRULENCE PLASMID
GULIG, PA
CALDWELL, AL
CHIODO, VA
[J]. MOLECULAR MICROBIOLOGY, 1992, 6 (10) : 1395 - 1411
[9] CLONING AND SEQUENCE-ANALYSIS OF GENES INVOLVED IN ERYTHROMYCIN BIOSYNTHESIS IN SACCHAROPOLYSPORA-ERYTHRAEA - SEQUENCE SIMILARITIES BETWEEN ERYG AND A FAMILY OF S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES
HAYDOCK, SF
DOWSON, JA
DHILLON, N
ROBERTS, GA
CORTES, J
LEADLAY, PF
[J]. MOLECULAR & GENERAL GENETICS, 1991, 230 (1-2): : 120 - 128
[10] TRANSPOSABLE ELEMENTS IN PROKARYOTES
KLECKNER, N
[J]. ANNUAL REVIEW OF GENETICS, 1981, 15 : 341 - 404

← 1 2 →