AMPLIFICATION AND DIRECT SEQUENCING OF A CDNA-ENCODING HUMAN CYTOSOLIC 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A SYNTHASE

被引：26

作者：

RUSS, AP ^{[1
]}

RUZICKA, V ^{[1
]}

MAERZ, W ^{[1
]}

APPELHANS, H ^{[1
]}

GROSS, W ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS,ZELLPHYSIOL,FRANKFURT,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1132卷 / 03期

关键词：

3-HYDROXY-3-METHYLGLUTARYL COA (HMG-COA) SYNTHASE CDNA; CHOLESTEROL BIOSYNTHESIS; ISOPRENOID BIOSYNTHESIS; (HUMAN);

D O I：

10.1016/0167-4781(92)90172-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosolic 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) synthase (E.C. 4.1.3.5) is a highly regulated enzyme involved in isoprenoid biosynthesis and therefore a potential target for cholesterol-lowering drugs. Up to now, primary structure data have only been available for chicken, rat and hamster HMG-CoA synthase. Using in vitro amplification and direct sequencing, we have determined the nucleotide sequence of the coding region of the human cytosolic 3-hydroxy-3-methylglutaryl CoA synthase cDNA.

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页码：329 / 331

页数：3

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