SEA BASS (DICENTRARCHUS-LABRAX L) VITELLOGENIN .1. INDUCTION, PURIFICATION AND PARTIAL CHARACTERIZATION

被引：56

作者：

MANANOS, E

ZANUY, S

LEMENN, F

CARRILLO, M

NUNEZ, J

机构：

[1] INST ACUICULTURA TORRE DE LA SAL, Castellon de La Plana, SPAIN

[2] UNIV BORDEAUX 1, BIOL MARINE LAB, F-33405 TALENCE, FRANCE

[3] ORSTOM, CTR RECH OCEANOL, ABIDJAN, COTE IVOIRE

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1994年 / 107卷 / 02期

关键词：

DICENTRARCHUS LABRAX; VITELLOGENIN;

D O I：

10.1016/0305-0491(94)90041-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In sea bass (males and non-vitellogenic females) estradiol (E(2)) treatment caused the appearance in the bloodstream of a new protein that was identified as the vitellogenin (VTG) molecule for this teleost species. The molecular weight of the sea bass VTG (445 kDa) and its amino acid composition was similar to that of other teleosts. VTG was purified by double-step chromatography and then used to obtain specific antibodies. The localization of VTG in the liver and in the ovary was carried out by immunocytochemistry. The effects of the E(2) treatment on the synthetic activity of the liver were studied throughout the modifications observed at the histological and biochemical levels.

引用

页码：205 / 216

页数：12

共 67 条

[1] CHEMICAL COMPOSITION OF AN OESTROGEN-INDUCED CALCIUM-BINDING GLYCOLIPOPHOSPHOPROTEIN IN XENOPUS-LAEVIS [J].