REGULATION OF TISSUE-SPECIFIC P-ELEMENT PRE-MESSENGER-RNA SPLICING REQUIRES THE RNA-BINDING PROTEIN-PSI

被引:100
作者
SIEBEL, CW
KANAAR, R
RIO, DC
机构
[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, DIV GENET, BERKELEY, CA 94720 USA
[2] UNIV CALIF BERKELEY, DIV BIOCHEM & MOLEC BIOL, BERKELEY, CA 94720 USA
关键词
ALTERNATIVE SPLICING; P ELEMENTS; PSI; HRP48; RNA BINDING PROTEINS; PURIFICATION;
D O I
10.1101/gad.8.14.1713
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Binding of a multiprotein complex to a 5' exon inhibitory element appears to repress splicing of the Drosophila P-element third intron (IVS3) in the soma. We have purified 97- and 50-kD proteins that interact specifically with the inhibitory element using RNA affinity chromatography. Antibodies specific for the 97-kD protein relieve inhibition of IVS3 splicing in somatic extracts, providing direct evidence that inhibition requires this protein, P-element somatic inhibitor (PSI). We identify the 50-kD protein as hrp48, a protein similar to the mammalian splicing factor hnRNP A1, and show that hrp48 recognizes specific nucleotides in a pseudo-5' splice site within the inhibitory element. The results indicate that PSI is an alternative splicing factor that regulates tissue-specific splicing, probably through interactions with generally expressed factors such as hrp48.
引用
收藏
页码:1713 / 1725
页数:13
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