EXPRESSION OF PCNA, BASIC FIBROBLAST GROWTH-FACTOR, FGF-RECEPTOR AND VASCULAR ENDOTHELIAL GROWTH-FACTOR IN ADENOMAS AND CARCINOMAS OF HUMAN COLON

被引：18

作者：

SHIRAISHI, A ^{[1
]}

ISHIWATA, T ^{[1
]}

SHOJI, T ^{[1
]}

ASANO, G ^{[1
]}

机构：

[1] NIPPON MED COLL, DEPT SURG 2, BUNKYO KU, TOKYO 113, JAPAN

来源：

ACTA HISTOCHEMICA ET CYTOCHEMICA | 1995年 / 28卷 / 01期

关键词：

HUMAN COLON CARCINOMA; PROLIFERATING CELL NUCLEAR ANTIGEN; BASIC FIBROBLAST GROWTH FACTOR; VASCULAR ENDOTHELIAL GROWTH FACTOR; IN SITU HYBRIDIZATION;

D O I：

10.1267/ahc.28.21

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study was designed to clarify the role of growth factors and vascular proliferation in the growth of colon tumor. On immunohistochemical observation, the ratios of positive cells for PCNA, bFGF and VEGF in carcinomas and adenomas were higher than in non-neoplastic mucosa. On reverse transcriptase polymerase chain reaction (RT-PCR) study, the expression of bFGF mRNA, FGF-receptor (FGFR) mRNA and VEGF mRNA was confirmed in all cases including non-neoplastic tissues, adenomas and carcinomas. On in situ hybridization, bFGF mRNA was expressed in carcinoma cells, vascular cells and fibroblastic cells, and the expression ratio of bFGF mRNA in carcinomas was higher than in non-carcinomatous mucosa. VEGF mRNA was expressed in carcinoma cells, medial smooth muscle cells and fibroblastic cells. These results suggest that bFGF and VEGF may contribute to tumor growth in association with neovascularization.

引用

页码：21 / 29

页数：9

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