MOLECULAR-CLONING OF A HUMAN BETA-3-ADRENERGIC RECEPTOR CDNA

被引：62

作者：

LELIAS, JM ^{[1
]}

KAGHAD, M ^{[1
]}

RODRIGUEZ, M ^{[1
]}

CHALON, P ^{[1
]}

BONNIN, J ^{[1
]}

DUPRE, I ^{[1
]}

DELPECH, B ^{[1
]}

BENSAID, M ^{[1
]}

LEFUR, G ^{[1
]}

FERRARA, P ^{[1
]}

CAPUT, D ^{[1
]}

机构：

[1] SANOFI ELF BIORECH,BIOL MOLEC LAB,BP 137,F-31676 LABEGE,FRANCE

来源：

FEBS LETTERS | 1993年 / 324卷 / 02期

关键词：

INTRON; ALTERNATIVE SPLICING; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0014-5793(93)81377-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the molecular cloning of a beta3-adrenergic receptor [beta3-AR] cDNA from human brown adipose tissue. The cDNA-encoded protein is identical to the previously cloned beta3-AR but with 6 additional amino acids at the C-terminus. The C-terminus is shared by the beta3 receptors expressed in human neuroblastoma cells [SK-N-MC] [Mol. Pharmacol. 42 (1992) 964 970]. Furthermore, using a polymerase chain reaction strategy we have cloned and sequenced the beta3-AR introns. Sequence analysis demonstrates that the human beta3-AR gene comprises at least 3 exons and 2 introns and that the most abundant beta3-AR transcripts encode a protein with an exon 3-derived C-terminus. Interestingly, although a similar organization has been found in rodent genes, the rat beta3-AR transcripts encode a receptor with an exon 2-derived C-terminus.

引用

页码：127 / 130

页数：4

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