COMPARATIVE SEQUENCE-ANALYSIS OF CIS-ELEMENTS PRESENT IN GLYCINE-MAX L LEGHEMOGLOBIN LBA AND LBC3 GENES

被引：17

作者：

SHE, QX ^{[1
]}

SANDAL, NN ^{[1
]}

STOUGAARD, J ^{[1
]}

MARCKER, KA ^{[1
]}

机构：

[1] AARHUS UNIV,DEPT MOLEC BIOL,GENE EXPRESS LAB,GUSTAV WIEDS VEJ 10,DK-8000 AARHUS,DENMARK

来源：

PLANT MOLECULAR BIOLOGY | 1993年 / 22卷 / 05期

关键词：

GLYCINE-MAX; LEGHEMOGLOBIN; GENE REGULATION; POSITIVE ELEMENTS; INVERTED REPEATS; ENHANCER MOTIFS;

D O I：

10.1007/BF00027380

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enhancer in the upstream positive elements. Within the minimal enhancer, an inverted repeat with similarity to the binding site of a yeast transcription factor, GCN4, was found. This particular repeat is conserved in the promoters of all functional soybean lb genes as well as in lb gene promoters from other legumes. This suggests that the inverted repeat is important for leghemoglobin gene expression.

引用

页码：931 / 935

页数：5

共 16 条

[1] THE CHROMOSOMAL ARRANGEMENT OF 6 SOYBEAN LEGHEMOGLOBIN GENES [J].

BOJSEN, K ;

ABILDSTEN, D ;

JENSEN, EO ;

PALUDAN, K ;

MARCKER, KA .

EMBO JOURNAL, 1983, 2 (07) :1165-1168

[2] 5' FLANKING SEQUENCE OF THE SOYBEAN LEGHEMOGLOBIN IBC3 GENE [J].

CHRISTENSEN, T ;

SANDAL, NN ;

STOUGAARD, J ;

MARCKER, KA .

NUCLEIC ACIDS RESEARCH, 1989, 17 (11) :4383-4383

[3] FOS AND JUN - THE AP-1 CONNECTION [J].

CURRAN, T ;

FRANZA, BR .

CELL, 1988, 55 (03) :395-397

[4] SEPARATION AND DETERMINATION OF THE RELATIVE CONCENTRATIONS OF THE HOMOGENEOUS COMPONENTS OF SOYBEAN LEGHEMOGLOBIN BY ISOELECTRIC-FOCUSING [J].

FUCHSMAN, WH ;

APPLEBY, CA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 579 (02) :314-324

[5]

GALLUSI P, 1991, THESIS U P SABATIER

[6]

HILSON P, 1990, PLANT CELL, V2, P651, DOI 10.1105/tpc.2.7.651

[7] INTERACTION OF A NODULE SPECIFIC, TRANS-ACTING FACTOR WITH DISTINCT DNA ELEMENTS IN THE SOYBEAN LEGHAEMOGLOBIN LBC3 5' UPSTREAM REGION [J].

JENSEN, EO ;

MARCKER, KA ;

SCHELL, J ;

DEBRUIJN, FJ .

EMBO JOURNAL, 1988, 7 (05) :1265-1271

[8] TRANSCRIPTION OF THE SOYBEAN LEGHEMOGLOBIN GENES DURING NODULE DEVELOPMENT [J].

MARCKER, A ;

LUND, M ;

JENSEN, EO ;

MARCKER, KA .

EMBO JOURNAL, 1984, 3 (08) :1691-1695

[9]

MARCKER KA, 1983, MOL GENETICS BACTERI, P149

[10] PRIMARY STRUCTURE AND PROMOTER ANALYSIS OF LEGHEMOGLOBIN GENES OF THE STEM-NODULATED TROPICAL LEGUME SESBANIA-ROSTRATA - CONSERVED CODING SEQUENCES, CIS-ELEMENTS AND TRANS-ACTING FACTORS [J].

METZ, BA ;

WELTERS, P ;

HOFFMANN, HJ ;

JENSEN, EO ;

SCHELL, J ;

DEBRUIJN, FJ .

MOLECULAR AND GENERAL GENETICS, 1988, 214 (02) :181-191

← 1 2 →