SEPARATION OF ENANTIOMERS BY CAPILLARY ELECTROPHORETIC TECHNIQUES

This review surveys the separation of enantiomers by capillary electrophoretic (CE) techniques for those who are not highly acquainted with CE techniques. The CE techniques described are capillary zone electrophoresis, electrokinetic chromatography, isotachophoresis, capillary gel electrophoresis and electrochromatography. The fundamental separation theory of each CE technique is discussed from the viewpoint of the optimization of selectivity and resolution. Enantiomeric separations by the CE techniques are mostly based on complex formation between the analytes and the chiral selectors added to the separation system, and therefore the chiral additives and their effects are discussed, including interaction models. The indirect separation of enantiomers by the prederivatization to diastereomers is also briefly discussed.