A HIGHLY STABLE AND SELECTIVE BIOSENSOR USING MODIFIED NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR)

被引：16

作者：

ERAY, M

DOGAN, NS

REIKEN, SR

SUTISNA, H

VANWIE, BJ

KOCH, AR

MOFFETT, DF

SILBER, M

DAVIS, WC

机构：

[1] WASHINGTON STATE UNIV,SCH ELECT ENGN & COMP SCI,PULLMAN,WA 99164

[2] WASHINGTON STATE UNIV,DEPT CHEM ENGN,PULLMAN,WA 99164

[3] WASHINGTON STATE UNIV,DEPT ZOOL,PULLMAN,WA 99164

[4] WASHINGTON STATE UNIV,DEPT VET MICROBIOL & PATHOL,PULLMAN,WA 99164

来源：

BIOSYSTEMS | 1995年 / 35卷 / 2-3期

关键词：

BIOSENSOR; MICROFABRICATION; BILAYER LIPID MEMBRANES; BISPECIFIC ANTIBODIES; MODIFIED NICOTINIC ACETYLCHOLINE RECEPTOR;

D O I：

10.1016/0303-2647(94)01511-5

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.

引用

页码：183 / 188

页数：6

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