A MEMBER OF THE CTF/NF-1 TRANSCRIPTION FACTOR FAMILY REGULATES MURINE GROWTH-HORMONE RECEPTOR GENE PROMOTER ACTIVITY

被引：18

作者：

ZOU, LL ^{[1
]}

MENON, RK ^{[1
]}

机构：

[1] UNIV PITTSBURGH, CHILDRENS HOSP PITTSBURGH, SCH MED, DEPT PEDIAT, PITTSBURGH, PA 15213 USA

来源：

ENDOCRINOLOGY | 1995年 / 136卷 / 11期

关键词：

D O I：

10.1210/en.136.11.5236

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Deletional analysis by transient transfection of COS-7 cells with the murine growth hormone (GH) receptor gene promoter for the L1 transcript identified a 16-bp enhancer element located approximately 3.0 kb upstream of the major transcription start sites. The sequence of this enhancer element suggested a binding motif for the CTF/NF-1 family of transcription factors. In electromobility shift assays (EMSA) this DNA-element formed a sequence-specific complex with nuclear proteins from COS-7 cells. Competition experiments with oligonucleotide containing a consensus binding motif for CTF/NF-1 protein(s) and supershift EMSA with an anti-CTF/NF-1 antibody established that a CTF/NF-1 like protein binds to this enhancer element.

引用

页码：5236 / 5239

页数：4

共 16 条

[1] NF1-L IS THE DNA-BINDING COMPONENT OF THE PROTEIN COMPLEX AT THE PERIPHERIN NEGATIVE REGULATORY ELEMENT [J].

ADAMS, AD ;

CHOATE, DM ;

THOMPSON, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (12) :6975-6983

[2] A RAPID MICROPREPARATION TECHNIQUE FOR EXTRACTION OF DNA-BINDING PROTEINS FROM LIMITING NUMBERS OF MAMMALIAN-CELLS [J].

ANDREWS, NC ;

FALLER, DV .

NUCLEIC ACIDS RESEARCH, 1991, 19 (09) :2499-2499

[3]

DU L, 1995, 77TH ANN M END SOC W, P342

[4] MULTIPLE GENES ENCODE NUCLEAR FACTOR-1-LIKE PROTEINS THAT BIND TO THE PROMOTER FOR 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE [J].

GIL, G ;

SMITH, JR ;

GOLDSTEIN, JL ;

SLAUGHTER, CA ;

ORTH, K ;

BROWN, MS ;

OSBORNE, TF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8963-8967

[5] AMINO-TERMINAL DOMAIN OF NF1 BINDS TO DNA AS A DIMER AND ACTIVATES ADENOVIRUS DNA-REPLICATION [J].

GOUNARI, F ;

DEFRANCESCO, R ;

SCHMITT, J ;

VANDERVLIET, PC ;

CORTESE, R ;

STUNNENBERG, H .

EMBO JOURNAL, 1990, 9 (02) :559-566

[6]

INOUE T, 1990, J BIOL CHEM, V265, P19065

[7] MODULATION OF LIVER-SPECIFIC TRANSCRIPTION BY INTERACTIONS BETWEEN HEPATOCYTE NUCLEAR FACTOR-III AND NUCLEAR FACTOR-I BINDING DNA IN CLOSE APPOSITION [J].

JACKSON, DA ;

ROWADER, KE ;

STEVENS, K ;

JIANG, CY ;

MILOS, P ;

ZARET, KS .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (04) :2401-2410

[8] A CELLULAR DNA-BINDING PROTEIN THAT ACTIVATES EUKARYOTIC TRANSCRIPTION AND DNA-REPLICATION [J].

JONES, KA ;

KADONAGA, JT ;

ROSENFELD, PJ ;

KELLY, TJ ;

TJIAN, R .

CELL, 1987, 48 (01) :79-89

[9] THE PROLACTIN GROWTH-HORMONE RECEPTOR FAMILY [J].

KELLY, PA ;

DJIANE, J ;

POSTELVINAY, MC ;

EDERY, M .

ENDOCRINE REVIEWS, 1991, 12 (03) :235-251

[10] CLONING OF THE PROMOTER-REGULATORY REGION OF THE MURINE GROWTH-HORMONE RECEPTOR GENE - IDENTIFICATION OF A DEVELOPMENTALLY-REGULATED ENHANCER ELEMENT [J].

MENON, RK ;

STEPHAN, DA ;

SINGH, M ;

MORRIS, SM ;

ZOU, LL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) :8851-8859

← 1 2 →