DETERMINATION OF ISOTOPE RATIOS IN HUMAN TISSUES ENRICHED WITH ZINC STABLE-ISOTOPE TRACERS USING INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY (ICP-MS)

被引：16

作者：

FRIEL, JK

LONGERICH, HP

JACKSON, SE

机构：

[1] MEM UNIV NEWFOUNDLAND,CTR EARTH RESOURCES RES,ST JOHNS A1B 3X9,NEWFOUNDLAND,CANADA

[2] MEM UNIV NEWFOUNDLAND,DEPT EARTH SCI,ST JOHNS A1B 3X9,NEWFOUNDLAND,CANADA

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 1993年 / 37卷 / 2-3期

关键词：

ZINC; STABLE ISOTOPES; ICP-MS; HUMAN STUDIES;

D O I：

10.1007/BF02783788

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enriched fecal and urine samples were prepared using ion-exchange column chromatography for analysis by Inductively Coupled and Fast Atom Bombardment Mass spectrometry (ICP-MS, FAB-MS) to compare precision between methods. Unenriched samples of human milk, feces, and whole blood were prepared similarly to monitor instrumental precision and analytical error. A least squares fit of the ICP-MS results vs the FAB-MS for Zn-70/Zn-64 gave a slope of 0.98, with a relative standard deviation (RSD) of only 0.7%. The results for Zn-68/Zn-64 gave a slope of 0.82, with a RSD of 14%. For unenriched tissues, all potential interferences were removed by the preparation procedure with no significant differences between preparation for isotope ratios of Zn-70/Zn-64, Zn-68/Zn-64, Zn-67/Zn-64, and Zn-66/Zn-64. Poisson counting statistics are a major contribution to the total analytical error indicating the usefulness of this procedure for enrichment studies.

引用

页码：123 / 136

页数：14

共 19 条

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BARNES R, 1991, AUG CAN SPECT SOC P

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