A DETERGENT-FREE METHOD FOR PURIFYING CAVEOLAE MEMBRANE FROM TISSUE-CULTURE CELLS

被引：651

作者：

SMART, EJ

YING, YS

MINEO, C

ANDERSON, RGW

机构：

[1] Dept. of Cell Biol. and Neuroscience, Univ. Texas Southwestern Med. Ctr., Dallas

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 22期

关键词：

G PROTEINS; EPIDERMAL GROWTH FACTOR RECEPTOR; FOLATE RECEPTOR;

D O I：

10.1073/pnas.92.22.10104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.

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页码：10104 / 10108

页数：5

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