TRANSFECTION OF HUMAN LAMIN-A AND LAMIN-C INTO MOUSE EMBRYONAL CARCINOMA-CELLS POSSESSING ONLY LAMIN-B

被引：19

作者：

COLLARD, JF ^{[1
]}

RAYMOND, Y ^{[1
]}

机构：

[1] HOP NOTRE DAME DE BON SECOURS, INST CANC MONTREAL, 1560 EST RUE SHERBROOKE, MONTREAL H2L 4M1, QUEBEC, CANADA

来源：

EXPERIMENTAL CELL RESEARCH | 1990年 / 186卷 / 01期

关键词：

D O I：

10.1016/0014-4827(90)90225-Y

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The peripheral lamina of eukaryotic nuclei is composed of polypeptides called lamins that vary in number from one to four according to organism, cell type, and differentiated state of the cells. Early embryonic cells and stem cells of mammals generally possess only lamin B while lamins A and C appear later during differentiation. To study the role of the late appearance of lamins A and C in the differentiated phenotype, we have performed transfection of cDNAs coding for human lamins A or C into mouse embryonal carcinoma (EC) cell lines F9 and P19 lacking these two lamins. Transient transfections have shown that lamins A or C could be expressed, translocated to the peripheral lamina, and distributed into daughter cell nuclei after mitosis. These results demonstrated that EC cells devoid of lamins A and C nevertheless possessed the appropriate mechanisms for the localization and mitotic redistribution of exogenous lamins A and C. © 1990.

引用

页码：182 / 187

页数：6

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