PROTEIN-KINASE-C OF A HUMAN MEGAKARYOBLASTIC LEUKEMIC-CELL LINE (MEG-01) - ANALYSIS OF SUBSPECIES AND ACTIVATION BY DIACYLGLYCEROL AND FREE FATTY-ACIDS

被引：10

作者：

KITAGAWA, Y

MATSUO, Y

MINOWADA, J

NISHIZUKA, Y

机构：

[1] KOBE UNIV,SCH MED,DEPT BIOCHEM,KOBE 650,JAPAN

[2] HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR,OKAYAMA 702,JAPAN

[3] KOBE UNIV,BIOSIGNAL RES CTR,KOBE 657,JAPAN

来源：

FEBS LETTERS | 1991年 / 288卷 / 1-2期

关键词：

PROTEIN KINASE-C; ARACHIDONIC ACID; MEGAKARYOCYTE; MEG-01-CELL;

D O I：

10.1016/0014-5793(91)80998-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein kinase C (PKC) from a human megakaryoblastic leukemic cell line (MEG-01) was resolved into two fractions by hydroxyapatite column chromatography, which are indistinguishable from the brain type II (beta-I/beta-II) and type III (alpha) subspecies, by biochemical and immunoblot analysis. In the presence of both phosphatidylserine and diacylglycerol, several free unsaturated fatty acids (FFA's), such as arachidonic, oleic, linoleic and linolenic acids, further enhanced the enzyme activation, and allowed the enzyme to exhibit almost full activity at nearly basal levels of Ca2+ concentration. The concentration of unsaturated FFA's giving rise to the maximum enzyme activation was around 2 x 10(-5) M. Palmitic and stearic acids were inactive. The result implies that, in addition to diacylglycerol, the receptor-mediated release of unsaturated FFA's from membrane phospholipids may also take part in the activation of PKC.

引用

页码：37 / 40

页数：4

共 21 条

[1] RECEPTOR-MEDIATED ACTIVATION OF PHOSPHOLIPASE-A2 VIA GTP-BINDING PROTEINS - ARACHIDONIC-ACID AND ITS METABOLITES AS 2ND MESSENGERS [J].