VISUALIZATION OF SEROTONIN EFFECTS ON RENAL VESSELS OF RATS

被引:32
作者
ENDLICH, K [1 ]
KUHN, R [1 ]
STEINHAUSEN, M [1 ]
DUSSEL, R [1 ]
机构
[1] UNIV HEIDELBERG,INST PHYSIOL 1,NEUENHEIMER FELD 326,W-6900 HEIDELBERG,GERMANY
关键词
D O I
10.1038/ki.1993.49
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
We studied the effects of serotonin (5-hydroxytryptamine, 5-HT) on glomerular blood flow (GBF) and on renal vessel diameters in the hydronephrotic kidney and in vascular casts of normal kidneys of rats. 5-HT (60 min after local application of 10(-8) mol . liter-1) constricted the arcuate arteries (-10 +/- 2% to -14 +/- 2%, mean +/- SEM), dilated the interlobular arteries (+13 +/- 2%) and afferent arterioles (+17 +/- 3%), and decreased GBF (-44 +/- 5%). In contrast to normal autoregulation, reduction of renal perfusion pressure after local application of 5-HT from 118 +/- 3 mm Hg by 10 and 20 mm Hg reduced GBF by 12 +/- 2% and 23 +/- 3%, respectively. The 5-HT2 antagonist, ritanserin (60 min after local application of 10(-6) mol . liter-1), dilated all preglomerular vessels and increased GBF. In the presence of ritanserin, 5-HT lost nearly all vascular effects. During infusion of 5-HT (5 mug . min-1 i.v. for 20 min) vascular reactions were similar to those under local application. After cyclooxygenase inhibition with indomethacin, infusion of 5-HT failed to constrict the arcuate arteries whereas vasodilation of the small preglomerular vessels remained unaffected. Analyzing vascular casts of normal kidneys we observed considerable vascular spasms and an average vasoconstriction of the interlobar arteries of 19 +/- 9% after i.v. infusion of 5-HT. We believe that 5-HT decreases GBF by 5-HT2 receptor-mediated constriction of the large renal vessels which are modulated by the prostaglandin system, whereas 5-HT dilates the small preglomerular vessels, most likely via 5-HT1-like receptors. Furthermore, our data indicate that 5-HT impairs the myogenic component of renal autoregulation in the low pressure range.
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页码:314 / 323
页数:10
相关论文
共 46 条
[1]   HEMODYNAMIC AND RENIN RESPONSES TO (+/-)-DOI, A SELECTIVE 5-HT2 RECEPTOR AGONIST, IN CONSCIOUS RATS [J].
ALPER, RH .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1990, 175 (03) :323-332
[2]  
ALSIP N L, 1991, American Journal of Physiology, V261, pH1525
[3]   PHARMACOLOGICAL PROFILE OF RITANSERIN - A VERY SPECIFIC CENTRAL SEROTONIN S2-ANTAGONIST [J].
AWOUTERS, F ;
NIEMEGEERS, CJE ;
MEGENS, AAHP ;
MEERT, TF ;
JANSSEN, PAJ .
DRUG DEVELOPMENT RESEARCH, 1988, 15 (01) :61-73
[4]   CHRONIC ADMINISTRATION OF KETANSERIN AND THE DEVELOPMENT OF 2-KIDNEY-2-CLIP GOLDBLATT RENOVASCULAR HYPERTENSION IN THE RAT [J].
BASSO, N ;
KURNJEK, ML ;
MIKULIC, L ;
TROLLIET, MR .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1987, 10 :S62-S64
[5]   DIFFERENTIAL LARGE AND SMALL VESSEL RESPONSES TO SEROTONIN IN THE DOG HINDLIMB INVIVO - ROLE OF THE 5HT2 RECEPTOR [J].
BLACKSHEAR, JL ;
ORLANDI, C ;
GARNIC, JD ;
HOLLENBERG, NK .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1985, 7 (01) :42-49
[6]  
BLACKSHEAR JL, 1991, J CARDIOVASC PHARM, V17, P68, DOI 10.1097/00005344-199101000-00010
[7]   SEROTONIN AND THE RENAL BLOOD-SUPPLY - ROLE OF PROSTAGLANDINS AND THE 5HT-2 RECEPTOR [J].
BLACKSHEAR, JL ;
ORLANDI, C ;
HOLLENBERG, NK .
KIDNEY INTERNATIONAL, 1986, 30 (03) :304-310
[8]   PROPOSALS FOR THE CLASSIFICATION AND NOMENCLATURE OF FUNCTIONAL RECEPTORS FOR 5-HYDROXYTRYPTAMINE [J].
BRADLEY, PB ;
ENGEL, G ;
FENIUK, W ;
FOZARD, JR ;
HUMPHREY, PPA ;
MIDDLEMISS, DN ;
MYLECHARANE, EJ ;
RICHARDSON, BP ;
SAXENA, PR .
NEUROPHARMACOLOGY, 1986, 25 (06) :563-576
[9]   VASOCONSTRICTOR AND NOREPINEPHRINE POTENTIATING ACTION OF 5-HYDROXYKYNURAMINE IN THE ISOLATED PERFUSED RAT-KIDNEY - INVOLVEMENT OF SEROTONIN RECEPTORS AND ALPHA-1-ADRENOCEPTORS [J].
CHARLTON, KG ;
JOHNSON, TD ;
CLARKE, DE .
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1984, 328 (02) :154-159
[10]   INVIVO AND INVITRO ACTIVITY OF SELECTIVE 5-HYDROXYTRYPTAMINE2 RECEPTOR ANTAGONISTS [J].
CONOLAN, S ;
QUINN, MJ ;
TAYLOR, DA .
BRITISH JOURNAL OF PHARMACOLOGY, 1986, 89 (01) :129-135