REGULATION OF IGF BINDING-PROTEINS BY FSH IN HUMAN LUTEINIZING GRANULOSA-CELLS

被引：9

作者：

ADACHI, T

IWASHITA, M

KUROSHIMA, A

TAKEDA, Y

机构：

[1] Department of Obstetrics and Gynecology, Tokyo Women's Medical College, Tokyo, 162

[2] Department of Obstetrics and Gynecology, Tokyo Women's Medical College Daini Hospital, Tokyo, 116

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 09期

关键词：

IGFBP-1; IGFBP-3; FSH; HUMAN LUTEINIZING GRANULOSA CELLS; PROTEASE;

D O I：

10.1007/BF02212589

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Objectives: To evaluate the regulation of the secretion of insulin-like growth factor (IGF) binding proteins (IGFBPs) by FSH in human luteinizing granulosa cells. Design and Results: Luteinizing granulosa cells were incubated with and without FSH. Levels of lCFBP-1 and -3 in the medium were measured by EIA and RIA, respectively and binding activities of these IGFBP were evaluated by Western ligand blot. FSH inhibited the secretion of IGFBP-1 dose dependently. FSH did not inhibit the secretion of immunoreactive IGFBP-3, but inhibited the binding activity of IGFBP-3. To assess the protease activity, I-125-IGFBP-3 was incubated with the cultured medium. I-125-IGFBP-3 degraded into small fragments when it was incubated with the cultured medium treated with FSH. Conclusions: FSH enhances the action of IGF-I in human granulosa cells by inhibiting the secretion of IGFBP-1 and the binding activity of IGFBP-3 by stimulating the proteolysis of IGFBP-3.

引用

页码：639 / 643

页数：5

共 24 条

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