RADICAL INTERMEDIATES IN THE COENZYME-B12 DEPENDENT METHYLMALONYL-COA MUTASE REACTION SHOWN BY ESR SPECTROSCOPY

被引：74

作者：

ZHAO, Y ^{[1
]}

SUCH, P ^{[1
]}

RETEY, J ^{[1
]}

机构：

[1] UNIV KARLSRUHE,INST ORGAN CHEM,LEHRSTUHL BIOCHEM,RICHARD WILLSTATTER ALLEE,W-7500 KARLSRUHE 1,GERMANY

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH | 1992年 / 31卷 / 02期

关键词：

D O I：

10.1002/anie.199202151

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Only after the addition of the substrate, methylmalonyl-CoA, to the binary complex of highly purified methylmalonyl-CoA mutase and coenzyme B12 is a clear ESR signal observed. This proves that radical intermediates that have only been postulated in the past are indeed involved in the enzymatic rearrangement of the carbon skeleton.

引用

页码：215 / 216

页数：2

共 13 条

[1]

BABIOR BM, 1972, J BIOL CHEM, V247, P4389

[2]

BARIOR BM, 1974, J BIOL CHEM, V249, P4537

[3]

BRODIE JD, 1972, FED PROC, V31, P1578

[4] VITAMIN B12S-PROMOTED MODEL REARRANGEMENT OF METHYLMALONATE TO SUCCINATE IS NOT A FREE-RADICAL REACTION [J].

CHOI, GY ;

CHOI, SC ;

GALAN, A ;

WILK, B ;

DOWD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :3174-3176

[5] DETECTION OF INTERMEDIATES DURING CONVERSION OF PROPANE-1,2-DIOL TO PROPIONALDEHYDE BY GLYCEROLDEHYDRASE, A COENZYME B12 DEPENDENT REACTION [J].

COCKLE, SA ;

DAVIES, SP ;

FOSTER, MA ;

WILLIAMS, RJP ;

HILL, HAO .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1972, 94 (01) :275-+

[6]

FINLAY TH, 1973, J BIOL CHEM, V248, P1285

[7]

ORMEJOHNSON WH, 1974, J BIOL CHEM, V249, P2338

[8]

PILBROW JR, 1979, VITAMIN B12, P505

[9]

RETEY J, UNPUB

[10]

Retey J., 1982, B12, VII, P357

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