Cis-Regulatory elements involved in tobacco mosaic virus (TMV)-inducible expression were identified in a tobacco PR-5 gene, encoding an acidic thaumatin-like protein. By fusing upstream sequences of the PR-5 gene to the GUS reporter gene and analysing transgenic plants containing these fusions for local and systemic induction of GUS activity by TMV, it was found that sequences between -1364 and -718 are involved in TMV induction of PR-5 gene expression.