PREPARATION AND ENZYMATIC-ACTIVITY OF POLY[(N-ACYLIMINO)ETHYLENE]-MODIFIED CATALASE

被引：57

作者：

MIYAMOTO, M ^{[1
]}

NAKA, K ^{[1
]}

SHIOZAKI, M ^{[1
]}

CHUJO, Y ^{[1
]}

SAEGUSA, T ^{[1
]}

机构：

[1] KYOTO UNIV, FAC ENGN, DEPT SYNTHET CHEM, KYOTO 606, JAPAN

来源：

MACROMOLECULES | 1990年 / 23卷 / 13期

关键词：

D O I：

10.1021/ma00215a001

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

New amphiphilic polymeric modifiers, i.e., carboxylic acid terminated poly[N-acetylimino)-ethylene] (3a) and poly[(N-propionylimino)ethylene] (3b), were prepared, each of which was coupled with bovine liver catalase [H2O:H2O2 oxidoreductase; EC 1.11.1.6] by using a carbodiimide. The catalytic activity of the resulting polymer-enzyme hybrid was measured in an aqueous solution, and the influences of the molecular weight of the modifier and of the extent of modification on the enzymatic activity were examined. The activity of the hybrid was also assayed in benzene and in chloroform. It was significant to find that the 3b-modified catalase showed an excellent catalytic activity in benzene in comparison with a poly(ethyleneglycol) (PEG) modified catalase. The rate of decomposition of H2O2 catalyzed by the 3b-modified catalase was about 20 times higher than the PEG-modified one. © 1990, American Chemical Society. All rights reserved.

引用

页码：3201 / 3205

页数：5

共 23 条

[1]

Aebi H., 1974, METHODS ENZYMATIC AN, P673, DOI [10.1016/B978-0-12-091302-2.50032-3, DOI 10.1016/B978-0-12-091302-2.50032-3]

[2] POLYETHYLENE-GLYCOL MODIFICATION OF CANDIDA-RUGOSA LIPASE [J].

BAILLARGEON, MW ;

SONNET, PE .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (11) :1812-1815

[3]

BUCKMANN AF, 1981, MAKROMOL CHEM, V182, P1379

[4] IMMOBILIZED MICROBIAL-CELLS [J].

FUKUI, S ;

TANAKA, A .

ANNUAL REVIEW OF MICROBIOLOGY, 1982, 36 :145-172

[5] RESOLUTION OF RACEMIC MIXTURES VIA LIPASE CATALYSIS IN ORGANIC-SOLVENTS [J].

KIRCHNER, G ;

SCOLLAR, MP ;

KLIBANOV, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (24) :7072-7076

[6] SYNTHESIS OF POLY(2-METHYL-2-OXAZOLINE) MACROMERS [J].

KOBAYASHI, S ;

KAKU, M ;

SAWADA, S ;

SAEGUSA, T .

POLYMER BULLETIN, 1985, 13 (05) :447-451

[7]

KOBAYASHI S, 1987, POLYM PREPN JPN, V36, pE341

[8]

KOBAYASHI S, 1984, RING OPENING POLYM, V2, P761

[9] RULES FOR OPTIMIZATION OF BIOCATALYSIS IN ORGANIC-SOLVENTS [J].

LAANE, C ;

BOEREN, S ;

VOS, K ;

VEEGER, C .

BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (01) :81-87

[10] POLYETHYLENE GLYCOL-MODIFIED PAPAIN CATALYZES PEPTIDE-BOND FORMATION IN BENZENE [J].

LEE, H ;

TAKAHASHI, K ;

KODERA, Y ;

OHWADA, K ;

TSUZUKI, T ;

MATSUSHIMA, A ;

INADA, Y .

BIOTECHNOLOGY LETTERS, 1988, 10 (06) :403-407

← 1 2 3 →