REMOVAL OF 3' UNTRANSLATED SEQUENCES DRAMATICALLY ENHANCES TRANSIENT EXPRESSION OF OVINE FOLLICLE-STIMULATING-HORMONE BETA GENE MESSENGER-RIBONUCLEIC-ACID

被引：6

作者：

MOUNTFORD, PS ^{[1
]}

BRANDON, MR ^{[1
]}

ADAMS, TE ^{[1
]}

机构：

[1] UNIV MELBOURNE,SCH VET SCI,CTR ANIM BIOTECHNOL,PARKVILLE,VIC 3052,AUSTRALIA

来源：

JOURNAL OF NEUROENDOCRINOLOGY | 1992年 / 4卷 / 06期

关键词：

FOLLICLE-STIMULATING HORMONE BETA; MESSENGER RIBONUCLEIC ACID; 3' UNTRANSLATED SEQUENCE;

D O I：

10.1111/j.1365-2826.1992.tb00215.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The influence of the 3' untranslated (3'-UT) region of the ovine follicle-stimulating hormone (FSH) beta mRNA on the level of transcript expression was studied. Only very low levels of FSH beta mRNA were detected following transient transfection of COS cells with a eukaryotic expression vector containing a full-length ovine FSH beta cDNA that includes 1.1 kilobases (kb) of 3'-UT. In contrast, deletion of all but 135 basepairs (bp) of the 3'-UT resulted in a striking increase in FSH beta mRNA expression following transfection of the truncated cDNA construct. These observations suggest that sequences within the 3'-UT of the ovine FSH beta mRNA may play a significant role in the post-transcriptional regulation of FSH beta expression.

引用

页码：655 / 658

页数：4

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