VECTORS FOR DETERMINING THE DIFFERENTIAL EXPRESSION OF GENES IN HETEROCYSTS AND VEGETATIVE CELLS OF ANABAENA SP STRAIN PCC-7120

被引:11
作者
BAUER, CC [1 ]
HASELKORN, R [1 ]
机构
[1] UNIV CHICAGO,DEPT MOLEC GENET & CELL BIOL,CHICAGO,IL 60637
关键词
D O I
10.1128/jb.177.11.3332-3336.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Plasmid vectors were constructed to study promoters of the cyanobacterium Anabaena sp, strain PCC 7120. Plasmid pCCBSelect contains the promoterless reporter genes in the order cat-nifHDK. In pCCBSelect/a, the nifHDK operon precedes the cat gene. Putative promoter sequences were cloned into a polylinker region upstream of the reporter genes, Activity itS in heterocysts was determined by complementation of a strain containing a deletion of the nifH gene. Activity in vegetative cells was determined by measuring resistance to chloramphenicol. The promoter of the nifHDK. operon was active only in heterocysts; the promoter of the nifJ gene was active only in iron-depleted medium; and the promoters of the psbB gene, the ntcA, gene, and a newly found transcription factor gene were all active in both cell types.
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页码:3332 / 3336
页数:5
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