EXPRESSION OF A BACTERIAL GENE IN TRANSGENIC PLANTS CONFERS RESISTANCE TO THE HERBICIDE PHENMEDIPHAM

被引：13

作者：

STREBER, WR

KUTSCHKA, U

THOMAS, F

POHLENZ, HD

机构：

[1] Biochemical Research, Hoechst-Schering AgrEvo GmbH, Berlin, D-13465

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 25卷 / 06期

关键词：

CARBAMATE HYDROLASE; DESMEDIPHAM; DETOXIFICATION; HERBICIDE RESISTANCE; PHENMEDIPHAM; TRANSGENIC PLANTS;

D O I：

10.1007/BF00014671

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tobacco plants were genetically engineered to express a detoxifying pathway for the herbicide phenmedipham. A gene from Arthrobacter oxidans strain P52 that encodes an enzyme catalysing the hydrolytic cleavage of the carbamate compound phenmedipham has recently been cloned and sequenced. The coding sequence was fused with a cauliflower mosaic virus 35S promoter and introduced into tobacco plants by Agrobacterium-mediated gene transfer. Transgenic plants expressing high levels of phenmedipham hydrolase exhibited resistance when sprayed with the herbicide at up to ten times the usual field application rate.

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收藏

页码：977 / 987

页数：11

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