THE AMINOPEPTIDASE N-ENCODING PEPN GENE OF STREPTOMYCES-LIVIDANS-66

被引：17

作者：

BUTLER, MJ

APHALE, JS

BINNIE, C

DIZONNO, MA

KRYGSMAN, P

SOLTES, GA

WALCZYK, E

MALEK, LT

机构：

[1] Cangene Corporation, Mississauga

来源：

GENE | 1994年 / 141卷 / 01期

关键词：

INTRACELLULAR PEPTIDASE; EXPRESSION SCREENING; CHROMOGENIC PEPTIDE SUBSTRATES; PROTEIN OVERPRODUCTION;

D O I：

10.1016/0378-1119(94)90137-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene (pepN) encoding an aminopeptidase N (PepN) has been cloned from Streptomyces lividans. This was done using either leucine-beta-naphthylamide or arginine-beta-naphthylamide in a liquid overlayer on colonies growing on agar medium to screen for overproduction of the ability to hydrolyse the substrates. The nucleotide sequence of pepN was determined and shown to encode a 95-kDa protein, which displayed significant homology to PepN proteins from other organisms. Analysis of the overproduced proteinase confirmed that this protein was located intracellularly as a monomeric active species. PepN is a metallo-exopeptidase cleaving next to Leu, Arg and Lys in peptide-bond-containing substrates.

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页码：115 / 119

页数：5

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