PMA-INDUCED DOWN-REGULATION OF THE RECEPTOR FOR ALPHA-2-MACROGLOBULIN IN HUMAN U937 CELLS

被引:17
作者
CONESE, M
CAVALLARO, U
SIDENIUS, N
OLSON, D
SORIA, MR
BLASI, F
机构
[1] HOSP SAN RAFFAELE,DIBIT,IST SCI,DEPT BIOL & TECHNOL RES,I-20132 MILAN,ITALY
[2] UNIV MILAN,DEPT GENET & MICROBIAL BIOL,I-20132 MILAN,ITALY
[3] UNIV COPENHAGEN,INST MICROBIOL,COPENHAGEN,DENMARK
关键词
PHORBOL ESTER; UROKINASE RECEPTOR; ALPHA-2-MACROGLOBULIN RECEPTOR; ENDOCYTOSIS;
D O I
10.1016/0014-5793(94)01399-L
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription and expression of the urokinase (uPA) receptor (uPAR) are strongly stimulated by PMA. As for uPAR, the expression of alpha 2-MR is regulated by PMA in U937 cells. Ligand blotting experiments with the 39 kDa receptor-associated protein RAP, a ligand for alpha 2-MR, indicated that alpha 2-MR levels first increased and then decreased after PMA treatment. FACscan as well as immunoblotting analysis with alpha 2-MR-specific antibodies showed an identical trend: alpha 2-MR levels increased within the first day of treatment with PMA, decreased at later times, and totally disappeared by three days of treatment. The effect of PMA was not due to transcriptional down-regulation, as the alpha 2-MR mRNA level did not decrease at later times. Sensitivity of U937 cells to uPA-saporin, a toxin conjugate that reguires binding to uPAR for killing activity, was also markedly decreased. These results suggest that uPAR-mediated endocytosis depends on alpha 2-MR expression.
引用
收藏
页码:73 / 78
页数:6
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