DEVELOPMENT OF DNA PROBES FOR THE IDENTIFICATION OF SIBLING SPECIES-A OF THE ANOPHELES-CULICIFACIES (DIPTERA, CULICIDAE) COMPLEX

被引：10

作者：

GUNASEKERA, MB

DESILVA, BGDNK

ABEYEWICKREME, W

SUBBARAO, SK

NANDADASA, HG

KARUNANAYAKE, EH

机构：

[1] UNIV SRI JAYEWARDENEPURA,FAC APPL SCI,NUGEGODA,SRI LANKA

[2] UNIV COLOMBO,FAC MED,DEPT PARASITOL,COLOMBO,SRI LANKA

[3] MALARIA RES CTR,DELHI,INDIA

[4] UNIV COLOMBO,FAC MED,DEPT BIOCHEM & MOLEC BIOL,COLOMBO,SRI LANKA

来源：

BULLETIN OF ENTOMOLOGICAL RESEARCH | 1995年 / 85卷 / 03期

关键词：

D O I：

10.1017/S0007485300036075

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Three highly repetitive DNA sequences Rp36, Rp217 and Rp234, have been isolated from Anopheles culicifacies Giles sensu late. The cloned DNA sequences were found at a higher copy number in species B and C, than in species A of the A. culicifacies complex. These sequences may therefore be used as DNA probes to distinguish species A from the other two species, using a 200-fold dilution of a single mosquito DNA extract in a dot-blot hybridization assay. Rp36 and Rp217 have been completely sequenced. Internal repeats were absent in Rp36. Two related core sequences of 13 and 16 bp were found tandemly repeated in Rp217. These probes enable the rapid detection of species A of A. culicifacies in field investigations.

引用

页码：345 / 353

页数：9

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