A NOVEL COMPOUND, DEPUDECIN, INDUCES PRODUCTION OF TRANSFORMATION TO THE FLAT PHENOTYPE OF NIH3T3 CELLS TRANSFORMED BY RAS-ONCOGENE

A novel compound, depudecin, induced production of the flat phenotype of Ki-ras-transformed NIH3T3 cells at the low concentration of 1 gmg/ml. This effect was reversible. Actin stress fiber was detected in these cells after depudecin treatment. Almost complete reversion to the flat phenotype was observed at 6 h after depudecin addition. The synthesis of ras-mRNA did not decrease enough with depudecin treatment at the concentration of 10 μg/ml to reverse the transformed morphology. © 1992 Academic Press, Inc.