MOLECULAR-CLONING AND CHARACTERIZATION OF THE MOUSE DOPAMINE D-3 RECEPTOR GENE - AN ADDITIONAL INTRON AND AN MESSENGER-RNA VARIANT

被引：17

作者：

FU, DY

SKRYABIN, BV

BROSIUS, J

ROBAKIS, NK

机构：

[1] CUNY,MT SINAI MED CTR,DEPT PSYCHIAT,NEW YORK,NY 10029

[2] CUNY,MT SINAI MED CTR,FISHBERG RES CTR NEUROBIOL,NEW YORK,NY 10029

来源：

DNA AND CELL BIOLOGY | 1995年 / 14卷 / 06期

关键词：

D O I：

10.1089/dna.1995.14.485

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intron-exon organization for the murine dopamine D-3 receptor gene was determined. A novel intron of approximately 1 kb was identified in both rat and mouse D-3 receptor genes. This intron (termed intron 4) is situated between coding nucleotides 723 and 724, resulting in a split of former exon 4 (containing nucleotides 527-801) into two separate exons (exon 4 and exon 5). Thus, the coding regions of the D-2 and D-3 receptor genes contain an identical number of exons (seven exons) and share a very similar gene structure. Reverse transcription-PCR experiments revealed a short form of mouse D-3 mRNA (D-3Short) that lacks the first 63 nucleotides from exon 6, and results from a splicing event occurring within this exon. However, this mRNA variant was not found in either rat or human brain. No dopamine D-3 receptor mRNA variants were found deriving from the alternative splicing of exon 5, although its counterpart, exon 6 in the D-2 receptor gene, is spliced out to produce the D-2Short mRNA. These data suggest that, although the intron-exon organizations of the D-2 and D-3 receptor genes are similar, the encoded transcripts may be processed differently.

引用

页码：485 / 492

页数：8

共 28 条

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