CYCLODEXTRINS AS PROTEIN-FOLDING AIDS

被引：133

作者：

KARUPPIAH, N ^{[1
]}

SHARMA, A ^{[1
]}

机构：

[1] CENT MICHIGAN UNIV, DEPT CHEM, MT PLEASANT, MI 48859 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 211卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1778

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aggregation of proteins is a frequent occurence during their transition from random coil to native structure. The influence of cyclodextrins in the refolding of carbonic anhydrase under aggregating conditions was studied. Cyclodextrin prevented formation of protein aggregates during renaturation of carbonic anhydrase. In addition, over 90 % of active enzyme was recovered even at protein concentrations as high as 67 mu M The enhanced protein reactivation by cyclodextrins may be due to their ability to bind to hydrophobic sites in protein folding intermediate(s) followed by their subsequent removal as the protein refolds. (C) 1995 Academic Press, Inc.

引用

页码：60 / 66

页数：7

共 13 条

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