A COMMON MOTIF OF 2 ADJACENT PHOSPHOSERINES IN BOVINE, RABBIT AND HUMAN CARDIAC TROPONIN-I

被引：64

作者：

MITTMANN, K ^{[1
]}

JAQUET, K ^{[1
]}

HEILMEYER, LMG ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,INST PHYSIOL CHEM,BIOCHEM SUPRAMOLEK SYST ABT,POSTFACH 102148,W-4630 BOCHUM,GERMANY

来源：

FEBS LETTERS | 1990年 / 273卷 / 1-2期

关键词：

Cardiac troponin I; Human heart; N-terminal sequence; Phosphorylation site; Phosphoserine; Rabbit heart;

D O I：

10.1016/0014-5793(90)81046-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From rabbit and human cardiac troponin I N-terminal mono and bisphosphorylated peptides were isolated which were obtained from Lys-C proteinase digests. Two adjacent phosphoserine residues could be localized in each phosphopeptide following further tryptic digestion. The previously published sequence of rabbit cardiac troponin I had to be corrected. Two adjacent phosphoserine residues are a common motif in the very similar sequences of bovine, rabbit and human cardiac troponin I. The N-terminal sequences are: AcADRSGGSTAG DTVPAPPPVR RRS(P)S(P) ANYRAY ATEPHAK (bovine), AcADESTDA-AG EARPAPAPVR RRSPSPANYRAY ATEPHAK (rabbit), (Ac,A,D/N,G,S,S,D/N,A,A,R) EPRPAPAPIR RRS(P)SP-NYRAY ATEPHAK (human). © 1990.

引用

页码：41 / 45

页数：5

共 17 条

[1] ISOLATION AND CHARACTERIZATION OF A HIGHLY PHOSPHORYLATED TROPONIN FROM BOVINE HEART
BEIER, N
JAQUET, K
SCHNACKERZ, K
HEILMEYER, LMG
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 176 (02): : 327 - 334
[2] RAPID ANALYSIS OF AMINO-ACIDS USING PRE-COLUMN DERIVATIZATION
BIDLINGMEYER, BA
COHEN, SA
TARVIN, TL
[J]. JOURNAL OF CHROMATOGRAPHY, 1984, 336 (01): : 93 - 104
[3] PHOSPHORYLATION OF TROPONIN-I FROM CARDIAC-MUSCLE
COLE, HA
PERRY, SV
[J]. BIOCHEMICAL JOURNAL, 1975, 149 (03) : 525 - 533
[4] CARDIAC-SPECIFIC TROPONIN-I RADIOIMMUNOASSAY IN THE DIAGNOSIS OF ACUTE MYOCARDIAL-INFARCTION
CUMMINS, B
AUCKLAND, ML
CUMMINS, P
[J]. AMERICAN HEART JOURNAL, 1987, 113 (06) : 1333 - 1344
[5] CORRELATION BETWEEN CONTRACTION AND PHOSPHORYLATION OF INHIBITORY SUBUNIT OF TROPONIN IN PERFUSED RAT-HEART
ENGLAND, PJ
[J]. FEBS LETTERS, 1975, 50 (01): : 57 - 60
[6] AMINO-ACID SEQUENCE OF RABBIT CARDIAC TROPONIN I
GRAND, RJA
WILKINSON, JM
MOLE, LE
[J]. BIOCHEMICAL JOURNAL, 1976, 159 (03) : 633 - 641
[7] AMINO-ACID SEQUENCE OF BOVINE CARDIAC TROPONIN-I
LESZYK, J
DUMASWALA, R
POTTER, JD
COLLINS, JH
[J]. BIOCHEMISTRY, 1988, 27 (08) : 2821 - 2827
[8] SEQUENCE-ANALYSIS OF PHOSPHOSERINE-CONTAINING PEPTIDES - MODIFICATION FOR PICOMOLAR SENSITIVITY
MEYER, HE
HOFFMANNPOSORSKE, E
KORTE, H
HEILMEYER, LMG
[J]. FEBS LETTERS, 1986, 204 (01) : 61 - 66
[9] QUANTITATIVE-DETERMINATION OF PHOSPHOSERINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AS THE PHENYLTHIOCARBAMYL-S-ETHYLCYSTEINE - APPLICATION TO PICOMOLAR AMOUNTS OF PEPTIDES AND PROTEINS
MEYER, HE
SWIDEREK, K
HOFFMANNPOSORSKE, E
KORTE, H
HEILMEYER, LMG
[J]. JOURNAL OF CHROMATOGRAPHY, 1987, 397 : 113 - 121
[10] SITES OF PHOSPHORYLATION OF RABBIT CARDIAC TROPONIN-I BY ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE-DEPENDENT PROTEIN-KINASE - EFFECT OF INTERACTION WITH TROPONIN-C
MOIR, AJG
PERRY, SV
[J]. BIOCHEMICAL JOURNAL, 1977, 167 (02) : 333 - 343

← 1 2 →