MECHANISM OF PHENOLIC ACTIVATION OF AGROBACTERIUM VIRULENCE GENES - DEVELOPMENT OF A SPECIFIC INHIBITOR OF BACTERIAL SENSOR RESPONSE SYSTEMS

被引：51

作者：

HESS, KM ^{[1
]}

DUDLEY, MW ^{[1
]}

LYNN, DG ^{[1
]}

JOERGER, RD ^{[1
]}

BINNS, AN ^{[1
]}

机构：

[1] UNIV PENN,DEPT BIOL,INST PLANT SCI,PHILADELPHIA,PA 19104

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 17期

关键词：

SIGNAL TRANSDUCTION; PLASMID GENE EXPRESSION; CROWN GALL; VIR-A; ALPHA-DEHYDRODICONIFERYL ALCOHOL GLYCOSIDE;

D O I：

10.1073/pnas.88.17.7854

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aglycone of the dihydrodiconiferyl alcohol glycosides, a series of phenolic growth factors able to substitute for some of the hormone requirements of tobacco cell division, are also potent inducers of virulence gene expression in Agrobacterium tumefaciens. However, these factors do not conform to the previously established structural requirements necessary for vir expression. Systematic evaluation of the structural requirements of these inducers has led to a model detailing the role of the phenolics in induction. With this model, a specific inhibitor of vir induction has been developed. This inhibitor does not affect the induction of other genes on the Ti plasmid but irreversibly blocks vir expression. The inhibitor has been used to show that the inducing phenolics must be constantly present to maintain expression of the vir regulon.

引用

页码：7854 / 7858

页数：5

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