CLONING AND SEQUENCE OF IS1000, A PUTATIVE INSERTION-SEQUENCE FROM THERMUS-THERMOPHILUS HB8

被引：20

作者：

ASHBY, MK ^{[1
]}

BERGQUIST, PL ^{[1
]}

机构：

[1] UNIV AUCKLAND,DEPT CELLULAR & MOLEC BIOL,PRIVATE BAG,AUCKLAND,NEW ZEALAND

来源：

PLASMID | 1990年 / 24卷 / 01期

关键词：

D O I：

10.1016/0147-619X(90)90020-D

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The complete nucleotide sequences of two copies of a putative insertion sequence IS1000 fromThermus thermophilus HB8 are presented. IS1000 is 1196 base pairs long, contains a long open reading frame which could code for a protein of 317 amino acids, and has imperfect terminal inverted repeats of 6 base pairs (confirmed by the terminal sequencing of 4.5 copies of IS1000), but does not cause a target site duplication. There are at least 6 copies of IS1000 in the genome ofT. thermophilus HB8. A search of the GEN-EMBL data base revealed that the putative 317 ammo acid protein had significant homology with open reading frames in the transposable elements IS110 ofStreptomyces coelicolor and IS492 ofPseudomonas atlantica. © 1990 Academic Press, Inc. All rights reserved.

引用

页码：1 / 11

页数：11

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