BEAUVERIA-BASSIANA PROTOPLAST REGENERATION AND TRANSFORMATION USING ELECTROPORATION

被引：6

作者：

PFEIFER, TA ^{[1
]}

KHACHATOURIANS, GG ^{[1
]}

机构：

[1] UNIV SASKATCHEWAN, DEPT APPL MICROBIOL & FOOD SCI, BIOINSECTICIDE RES LAB, SASKATOON S7N 0W0, SASKATCHEWAN, CANADA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1992年 / 38卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Regeneration of Beauveria bassiana GK2016 protoplasts demonstrated three phases: (1) enlargement, (2) formation of a chain of budding cells, and (3) development of a true germ tube from the original protoplast. Regeneration frequencies of up to 80% were obtained when using the stabilizer ammonium sulphate. Using electroporation, protoplasts were transformed to methyl 1,2-benzimidazole carbamate (MBC) resistance with the Neurospora crassa MBC-resistant beta-tubulin gene. A transformation efficiency of one to three transformants per 5 mug vector DNA was obtained. The MBC phenotype was stable and transformants grew in the presence of 5 mug MBC/ml. Southern DNA-DNA hybridization analysis demonstrated that integration of the vector into the chromosomal DNA had occurred.

引用

页码：376 / 381

页数：6

共 29 条

[1]

BECKER DM, 1991, METHOD ENZYMOL, V194, P182

[2] A HIGH-EFFICIENCY TRANSFORMATION SYSTEM FOR THE BASIDIOMYCETE USTILAGO-VIOLACEA EMPLOYING HYGROMYCIN RESISTANCE AND LITHIUM-ACETATE TREATMENT [J].