DETERMINATION OF CLENBUTEROL IN BOVINE LIVER BY ENZYME-IMMUNOASSAY

被引：22

作者：

BUCKNALL, SD

MACKENZIE, AL

SAUER, MJ

EVEREST, DJ

NEWMAN, R

JACKMAN, R

机构：

[1] Biochemistry Discipline, Central Veterinary Laboratory, Weybridge, Surrey KT15 3NB, New Haw

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 275卷 / 1-2期

关键词：

ENZYMATIC METHODS; IMMUNOASSAY; BETA-AGONISTS; BOVINE LIVER; CLENBUTEROL; ELISA METHOD;

D O I：

10.1016/0003-2670(93)80296-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Procedures are described for optimising and validating an ELISA method for measuring clenbuterol residues in bovine liver without prior sample enrichment. Optimal assay conditions are obtained by preincubating liver supernatant with an immobilised antibody raised to clenbuterol-ovalbumen. After further incubation with a salbutamol-peroxidase conjugate, colour is developed using a tetramethylbenzidene substrate. The assay will permit measurement of clenbuterol residues in liver at the maximum residue level of 0.5 ng g-1 with a confidence of > 99%.

引用

页码：227 / 230

页数：4

共 4 条

[1] SALBUTAMOL RADIOIMMUNOASSAY - SYNTHESIS AND PROPERTIES OF THE BENZYLIC SUCCINATE OF SALBUTAMOL
BEAULIEU, N
CHARETTE, C
LOO, JCK
JORDAN, N
MCGILVERAY, IJ
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1985, 3 (06) : 575 - 579
[2] CONRADIE JD, 1983, J IMMUNOL METHODS, V59, P285
[3] Erlanger B F, 1980, Methods Enzymol, V70, P85
[4] DETERMINATION OF CLENBUTEROL IN BOVINE URINE BY ENZYME-IMMUNOASSAY FOLLOWING CONCENTRATION AND CLEANUP BY IMMUNOAFFINITY CHROMATOGRAPHY
PICKETT, RJH
SAUER, MJ
[J]. ANALYTICA CHIMICA ACTA, 1993, 275 (1-2) : 269 - 273

← 1 →