CHARACTERIZATION AND EXPRESSION OF A P-450-LIKE MYCINAMICIN BIOSYNTHESIS GENE USING A NOVEL MICROMONOSPORA ESCHERICHIA-COLI SHUTTLE COSMID VECTOR

被引：48

作者：

INOUYE, M ^{[1
]}

TAKADA, Y ^{[1
]}

MUTO, N ^{[1
]}

BEPPU, T ^{[1
]}

HORINOUCHI, S ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT BIOTECHNOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 245卷 / 04期

关键词：

MICROMONOSPORA GRISEORUBIDA; MYCINAMICIN BIOSYNTHESIS; SHUTTLE VECTOR; NUCLEOTIDE SEQUENCE; P-450;

D O I：

10.1007/BF00302258

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 29 kb shuttle cosmid vector, pTYS507, was constructed from a cryptic Micromonospora griseorubida plasmid and the Escherichia coli cosmid pJB8. Subcloning of mycinamicin II biosysnthesis genes in pTYS507 led to the identification of a DNA region that could complement a mutant of M. griseorubida that lacked both hydroxylase and epoxidase activities. Nucleotide sequence and mutational analysis suggested that a single P-450-like protein catalyzes both reactions.

引用

页码：456 / 464

页数：9

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