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PROSPECTIVE COMPARISON OF DIRECT IMMUNOFLUORESCENCE AND CONVENTIONAL STAINING METHODS FOR DETECTION OF GIARDIA AND CRYPTOSPORIDIUM SPP IN HUMAN FECAL SPECIMENS
被引：55
作者：
ALLES, AJ
WALDRON, MA
SIERRA, LS
MATTIA, AR
机构：
[1] MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114
[2] MASSACHUSETTS GEN HOSP,CLIN MICROBIOL LAB,BOSTON,MA 02114
关键词：
D O I：
10.1128/JCM.33.6.1632-1634.1995
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ;
100705 ;
摘要：
In a prospective comparative study, 2,696 consecutive fresh stool specimens over the course of 1 year were examined for Giardia lamblia and Cryptosporidium parvum by using a direct immunofluorescent-monoclonal antibody stain (for unspun specimens) and conventional staining methods (chlorazol black E for Giardia cysts and modified Kinyoun acid-fast for Cryptosporidium oocysts). The direct immunofluorescent-monoclonal antibody method resulted in a significantly increased detection rate for both giardia (118 versus 79 specimens, 49.4%; P = 0.006) and cryptosporidia (39 versus 23 specimens, 69.6%; P = 0.055).
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页码：1632 / 1634
页数：3
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