FLP RECOMBINASE IS AN ENZYME

被引：38

作者：

GATES, CA

COX, MM

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1988年 / 85卷 / 13期

关键词：

D O I：

10.1073/pnas.85.13.4628

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The FLP protein of the yeast 2-.mu.m plasmid catalyzes intermolecular site-specific recombination with a turnover number of .apprxeq. 0.12 min-1 (per FLP monomer) for relaxed DNA substrates. Under conditions that enhance its stability, the protein can be used in catalylic rather than stoichiometric amounts. The reaction rate rate exhibits a strong dependence on FLP protein concentration even when the protein is present in excess relative to available recombination sites.

引用

页码：4628 / 4632

页数：5

共 36 条

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