INTERFERON-GAMMA REDUCES ACTIN-FILAMENTS AND INHIBITS THYROID-STIMULATING HORMONE-INDUCED FORMATION OF MICROVILLI AND PSEUDOPODS IN MOUSE MONOLAYER THYROCYTES

被引：15

作者：

ASAKAWA, H

MIYAGAWA, JI

HANAFUSA, T

KATSURA, H

MIYAZAKI, A

OTSUKA, A

NAKAGAWA, C

YAMAGATA, K

TAJIMA, K

MASHITA, K

KONO, N

TARUI, S

机构：

[1] Second Department of Internal Medicine, Osaka University Medical School, Osaka

[2] Second Department of Internal Medicine, Osaka University Medical School, Fukushima-ku, Osaka, 553, 1-1-50, Fukushima

来源：

ENDOCRINOLOGY | 1990年 / 127卷 / 01期

关键词：

D O I：

10.1210/endo-127-1-325

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To study the role of interferon-γ (IFN γ), which is released from activated lymphocytes in the development of lymphocytic thyroiditis, we investigated the effects of IFN γ on the morphology of mouse thyrocytes, using scanning electron microscopy. Thyrocytes were cultured in monolayers for 5-7 days and incubated with TSH (10 mU/ml) for 1 h before fixation. IFN γ (200 U/ml) was added to the culture medium for 1 h to 5 days before TSH stimulation. Coculture of thyrocytes with IFN7 for more than 24 h inhibited TSH-induced morphological changes in the thyrocytes; IFN γ inhibited the increase in the number of and elongation of microvilli and the appearance of pseudopods. IFN γ also reduced the number of actin filaments in thyrocytes, as confirmed by immunofluorescence photometry. These results suggest that IFN γ inhibits the morphological response of thyrocytes to TSH stimulation by the reduction of actin filaments. © 1990 by The Endocrine Society.

引用

页码：325 / 329

页数：5

共 13 条

[1] ORGAN-SPECIFIC AUTOIMMUNITY - A 1986 OVERVIEW [J].