VIMENTIN IN HUMAN ERYTHROLEUKEMIA (HEL) CELLS IS MODULATED WITH DIFFERENTIATION INDUCERS

The expression of vimentin intermediate filaments (IFs) was studied in a human erythroleukemia cell line (HEL), exposed to a variety of differentiation-inducing agents. These cells grow normally in suspension and show a heterogenous expression of vimentin immunoreactivity. In the presence of retinoic acid the fibrillar vimentin immunoreactivity diminished rapidly, while it was increased when the cells were exposed to hemin or butyric acid. In the presence of a tumor promoter (TPA), the HEL cells maintained their heterogenous vimentin immunoreactivity, but some cells showed large bundles of cytoplasmic vimentin fibrils. Upon exposure to TPA the cells spread on a growth substratum covered with human plasma fibronectin (Fn). Many of the spread cells totally lacked vimentin IFs. The present results show that vimentin expression in HEL cells is rapidly and differentially modulated upon exposure to the different inducing agents. © 1990 Academic Press Ltd.