CLONING AND FUNCTIONAL EXPRESSION OF HUMAN CDNA FOR THE ETB ENDOTHELIN RECEPTOR

被引：272

作者：

SAKAMOTO, A

YANAGISAWA, M

SAKURAI, T

TAKUWA, Y

YANAGISAWA, H

MASAKI, T

机构：

[1] UNIV TSUKUBA,INST CLIN MED,SAKURA,IBARAKI 305,JAPAN

[2] UNIV TOKYO,DEPT INTERNAL MED 2,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)90158-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning of human cDNA encoding an ETB (non-isopeptide-selective) subtype of the endothelin receptor. The predicted amino acid sequence of the human ETB endothelin receptor was 87.8% and 62.9% identical with the previously cloned rat ETB and ETA receptors, respectively. COS cells transiently transfected with the cloned cDNA expressed specific, high-affinity binding sites for endothelin isopeptides and responded to the peptides with a transient increase of [Ca2+]i; endothelin-1 and endothelin-3 exhibited approximately equal potencies both in displacing 125I-labeled endothelin-1 binding and in eliciting [Ca2+]i transients. The ETB receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo. © 1991.

引用

页码：656 / 663

页数：8

共 29 条

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