COMPARISON OF ELASTIN PEPTIDE CONCENTRATIONS IN BODY-FLUIDS FROM HEALTHY-VOLUNTEERS, SMOKERS, AND PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY-DISEASE

被引:71
作者
SCHRIVER, EE
DAVIDSON, JM
SUTCLIFFE, MC
SWINDELL, BB
BERNARD, GR
机构
[1] VANDERBILT UNIV, MED CTR,SCH MED,DEPT MED,CTR LUNG RES, B-1317 MED CTR,1161 21ST AVE S, NASHVILLE, TN 37232 USA
[2] VANDERBILT UNIV, MED CTR, SCH MED, DEPT PATHOL, CTR LUNG PATHOL, NASHVILLE, TN 37232 USA
[3] VET ADM MED CTR, NASHVILLE, TN 37203 USA
来源
AMERICAN REVIEW OF RESPIRATORY DISEASE | 1992年 / 145卷 / 04期
关键词
D O I
10.1164/ajrccm/145.4_Pt_1.762
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Proteolysis of elastic fibers is central to the development of emphysema, and a simple, noninvasive assay of elastin degradation would be useful in diagnosis and in therapeutic monitoring. We have adapted an indirect enzyme-linked immunosorbent assay (ELISA) to determine plasma, urine, and bronchoalveolar lavage fluid (BALF) elastin peptide concentrations in nonsmokers, healthy smokers, and patients with chronic obstructive pulmonary disease (COPD). Plasma elastin peptide concentrations were significantly higher in subjects with COPD (66.8 +/- 5.8 ng/ml, n = 10) compared with nonsmokers (23.4 +/- 4.6 ng/ml, n = 12), and healthy smokers had intermediate values (36.0 +/- 6.8, n = 6), p < 0.05. Urine values (both unadjusted and normalized to urine creatinine concentration) were approximately 10-fold higher than plasma in all subject groups, and the relative differences among groups were the same as for plasma, with values of 910.8 +/- 105.6, 358.1 +/- 101.2, and 281.0 +/- 67.8 ng/ml for subjects with COPD (n = 10), healthy smokers (n = 6), and healthy nonsmokers (n = 12), respectively. Poor recovery of BALF in COPD subjects reduced differences in the BALF elastin peptide concentrations among subject groups, although the healthy smokers and COPD subjects tended to have higher amounts. Assuming some dilution due to lavage technique, elastin peptide concentrations were estimated to be substantially higher in epithelial lining fluid than in plasma, suggesting lung as a significant source of elastin peptides in COPD. This is the first application of elastin peptide measurement to human urine or BALF, and we conclude that this assay in urine is useful in characterizing elastin turnover in patients with or at risk for emphysema.
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页码:762 / 766
页数:5
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