CHARACTERIZATION OF AN ENDOTHELIAL PROTEIN IN THE DEVELOPING RABBIT KIDNEY

被引：10

作者：

KLOTH, S ^{[1
]}

MEYER, D ^{[1
]}

ROCKL, W ^{[1
]}

MIETTINEN, A ^{[1
]}

AIGNER, J ^{[1
]}

SCHMIDBAUER, A ^{[1
]}

MINUTH, WW ^{[1
]}

机构：

[1] UNIV HELSINKI,DEPT BACTERIOL & IMMUNOL,SF-00100 HELSINKI 10,FINLAND

来源：

DIFFERENTIATION | 1992年 / 52卷 / 01期

关键词：

ANGIOGENESIS; ENDOTHELIAL PROTEINS; KIDNEY DEVELOPMENT; MONOCLONAL ANTIBODY; RABBIT;

D O I：

10.1111/j.1432-0436.1992.tb00502.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A new protein (EnPo 1 antigen) abundant on endothelial cells and glomerular podocytes has been characterized by means of the mouse monoclonal antibody EnPo 1. Following electrophoretical separation of rabbit kidney homogenates EnPo 1 recognized a protein with a molecular weight of 110 kDa and an isoelectric point of 5.9 in Western blots. Using immunohistological techniques, the EnPo 1 antigen has been localized in high concentrations on glomerular podocytes of different developmental stages. Furthermore, the EnPo 1 antigen was expressed on endothelial cells of all adult rabbit organs tested so far. Detailed analysis of neonatal rabbit kidney revealed the abundance of EnPo 1 antigen on both differentiated vessels as well as on immature endothelial cells and endothelium of the microvasculature. Thus, for the first time a marker for in situ investigations of angiogenic processes within the mammalian kidney is available. Analysis of kidney cryosections by confocal laser scan microscopy revealed a direct connection between mature and differentiating vessels in the outer kidney cortex. Furthermore, two differentially organized cell populations discriminated by their EnPo 1 binding pattern were localized in the embryonic renal cortex. Morphologically, these cells were not distinguishable from other mesenchymal cells.

引用

页码：79 / 88

页数：10

共 29 条

[1]

ABRAHAMSON DR, 1991, SEMIN NEPHROL, V11, P375

[2]

BARD JBL, 1991, DEVELOPMENT, V113, P193

[3] MORPHOLOGICAL EVIDENCE THAT A-CAM IS A MAJOR INTERCELLULAR-ADHESION MOLECULE IN HUMAN KIDNEY [J].

BIDDLESTONE, LR ;

FLEMING, S .

JOURNAL OF PATHOLOGY, 1991, 164 (01) :9-15

[4] FORMATION OF BASEMENT-MEMBRANES IN THE EMBRYONIC KIDNEY - AN IMMUNOHISTOLOGICAL STUDY [J].

EKBLOM, P .

JOURNAL OF CELL BIOLOGY, 1981, 91 (01) :1-10

[5] THE ORIGIN OF THE GLOMERULAR ENDOTHELIUM [J].

EKBLOM, P ;

SARIOLA, H ;

KARKINENJAASKELAINEN, M ;

SAXEN, L .

CELL DIFFERENTIATION, 1982, 11 (01) :35-39

[6] ISOLATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES REACTIVE WITH ENDOTHELIAL-CELLS [J].

HAMBURGER, AW ;

REID, YA ;

PELLE, BA ;

BRETH, LA ;

BEG, N ;

RYAN, U ;

CINES, DB .

TISSUE & CELL, 1985, 17 (04) :451-459

[7] DIPEPTIDYL PEPTIDASE (DPP)-IV IN RAT ORGANS - COMPARISON OF IMMUNOHISTOCHEMISTRY AND ACTIVITY HISTOCHEMISTRY [J].

HARTEL, S ;

GOSSRAU, R ;

HANSKI, C ;

REUTTER, W .

HISTOCHEMISTRY, 1988, 89 (02) :151-161

[8] MONOCLONAL-ANTIBODY 5.5 REACTS WITH P8,14, A MYELOID MOLECULE ASSOCIATED WITH SOME VASCULAR ENDOTHELIUM [J].

HOGG, N ;

ALLEN, C ;

EDGEWORTH, J .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (06) :1053-1061

[9]

JOKELAINEN P, 1963, ACTA ANATOMICA S, V47, P6

[10] LY-6C IS A MONOCYTE MACROPHAGE AND ENDOTHELIAL-CELL DIFFERENTIATION ANTIGEN REGULATED BY INTERFERON-GAMMA [J].

JUTILA, MA ;

KROESE, FGM ;

JUTILA, KL ;

STALL, AM ;

FIERING, S ;

HERZENBERG, LA ;

BERG, EL ;

BUTCHER, EC .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (11) :1819-1826

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