THE CHOLINE TRANSPORTER IS THE MAJOR SITE OF CONTROL OF CHOLINE OXIDATION IN ISOLATED RAT-LIVER MITOCHONDRIA

被引：21

作者：

KAPLAN, CP ^{[1
]}

PORTER, RK ^{[1
]}

BRAND, MD ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD,CAMBRIDGE CB2 1QW,ENGLAND

来源：

FEBS LETTERS | 1993年 / 321卷 / 01期

基金：

英国惠康基金;

关键词：

METABOLIC CONTROL ANALYSIS; MITOCHONDRION; CHOLINE TRANSPORTER; CHOLINE OXIDATION; BETAINE;

D O I：

10.1016/0014-5793(93)80613-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The degree of control exerted by the mitochondrial choline transporter over the choline oxidation pathway was measured in isolated rat liver mitochondria. Choline transporter activity was titrated with hemicholinium-3, a known competitive inhibitor of the transporter. It was shown that the rate of betaine efflux from mitochondria was an accurate measure of choline oxidation. The relative rate of choline oxidation was measured as a function of the relative degree of inhibition of the transporter. The resulting data gave a flux control coefficient over choline oxidation of 0.9 for the choline transporter. It is concluded that the choline transporter is the major site for control of choline oxidation in isolated rat liver mitochondria.

引用

页码：24 / 26

页数：3

共 21 条

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