ISOLATION AND PREVALIDATION OF AN ESCHERICHIA-COLI TESTER STRAIN FOR THE USE IN MECHANISTIC AND METABOLIC STUDIES OF GENOTOXINS

被引：11

作者：

KRANENDONK, M ^{[1
]}

RUAS, M ^{[1
]}

LAIRES, A ^{[1
]}

RUEFF, J ^{[1
]}

机构：

[1] UNIV NOVA LISBOA, FAC SCI & TECHNOL, MONTE DE CAPARICA, PORTUGAL

来源：

MUTATION RESEARCH | 1994年 / 312卷 / 02期

关键词：

ESCHERICHIA-COLI; TESTER STRAIN;

D O I：

10.1016/0165-1161(94)90014-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have isolated an Escherichia coli tester strain for the use in mechanistic and metabolic studies of genotoxins. We started with one of the more used and better characterized E. coli K-12 laboratory strains, AB1157. We isolated a lipopolysaccharide defective mutant of strain AB1886 which is an excision repair deficient derivative of AB1157 and introduced a newly constructed plasmid pKR11, encoding mucAB, resulting in strain MR2101/pKR11. A genotoxicity assay was designed, monitoring the reversion to arginine prototrophy and a preliminary validation was carried out against Ames tester strain TA100 with a set of diagnostic compounds. The results seem to indicate that strain MR2101/pKR11 is an adequate tester strain which can be a useful tool in mechanistic studies. Moreover, this strain can serve as mother strain to isolate improved and more specialized tester strains.

引用

页码：99 / 109

页数：11

共 47 条

[1] IMPROVED BACTERIAL TEST SYSTEM FOR DETECTION AND CLASSIFICATION OF MUTAGENS AND CARCINOGENS [J].

AMES, BN ;

LEE, FD ;

DURSTON, WE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (03) :782-786

[2] LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8 [J].

BACHMANN, BJ .

MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :130-197

[3] INDUCTION OF -2 FRAMESHIFT MUTATIONS WITHIN ALTERNATING GC SEQUENCES BY CARCINOGENS THAT BIND TO THE C8 POSITION OF GUANINE RESIDUES - DEVELOPMENT OF A SPECIFIC MUTATION ASSAY [J].

BINTZ, R ;

FUCHS, RPP .

MOLECULAR & GENERAL GENETICS, 1990, 221 (03) :331-338

[4]

CALLEN DF, 1978, MUTAT RES, V55, P153, DOI 10.1016/0165-1110(78)90002-7

[5] COEXPRESSION OF UMUD' WITH UMUC SUPPRESSES THE UV MUTAGENESIS DEFICIENCY OF GROE MUTANTS [J].

DONNELLY, CE ;

WALKER, GC .

JOURNAL OF BACTERIOLOGY, 1992, 174 (10) :3133-3139

[6] INCREASED SENSITIVITY OF ESCHERICHIA-COLI-K12 TO CERTAIN MUTAGENS AS A CONSEQUENCE OF A MUTATION LEADING TO PHAGE U3 RESISTANCE [J].

ELLENBERGER, J .

MUTATION RESEARCH, 1982, 104 (1-3) :55-60

[7] CDNA-EXPRESSED HUMAN CYTOCHROME-P450S - A NEW AGE OF MOLECULAR TOXICOLOGY AND HUMAN RISK ASSESSMENT [J].

GONZALEZ, FJ ;

CRESPI, CL ;

GELBOIN, HV .

MUTATION RESEARCH, 1991, 247 (01) :113-127

[8] RECENT DEVELOPMENTS IN BACTERIAL SHORT-TERM TESTS FOR THE DETECTION OF GENOTOXIC AGENTS [J].

HOFNUNG, M ;

QUILLARDET, P .

MUTAGENESIS, 1986, 1 (05) :319-330

[9]

HOWARDFLANDERS P, 1966, GENETICS, V53, P1119

[10] THE SALMONELLA-TYPHIMURIUM MAMMALIAN MICROSOMAL ASSAY - A REPORT OF THE UNITED-STATES-ENVIRONMENTAL-PROTECTION-AGENCY GENE-TOX PROGRAM [J].

KIER, LE ;

BRUSICK, DJ ;

AULETTA, AE ;

VONHALLE, ES ;

BROWN, MM ;

SIMMON, VF ;

DUNKEL, V ;

MCCANN, J ;

MORTELMANS, K ;

PRIVAL, M ;

RAO, TK ;

RAY, V .

MUTATION RESEARCH, 1986, 168 (02) :69-240

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