PREPARATION OF 3'-PHOSPHOADENOSINE-5'-PHOSPHO S-35 SULFATE USING ATP SULFURYLASE AND APS KINASE FROM BACILLUS-STEAROTHERMOPHILUS - ENZYMATIC-SYNTHESIS AND PURIFICATION

Radioactive PAPS was synthesized with a predetermined specific activity using enzymes purified from Bacillus stearothermophilus. The reaction system, incorporating 1.5 mCi of S-35-H2SO4 and 500 nmol of ATP, produced 582 muCi of S-35-PAPS. The synthesized S-35-PAPS was purified by ECTEOLA cellulose chromatography, followed by desalting with DOWEX 50W-X8. The final yield was 206 mumol of S-35-PAPS with a radioactivity of 411 muCi.