CRY-J-I, A MAJOR ALLERGEN OF JAPANESE CEDAR POLLEN, HAS PECTATE LYASE ENZYME-ACTIVITY

被引：99

作者：

TANIGUCHI, Y ^{[1
]}

ONO, A ^{[1
]}

SAWATANI, M ^{[1
]}

NANBA, M ^{[1
]}

KOHNO, K ^{[1
]}

USUI, H ^{[1
]}

KURIMOTO, M ^{[1
]}

MATUHASI, T ^{[1
]}

机构：

[1] OKINAKA MEM INST MED RES,TORANOMON 105,JAPAN

来源：

ALLERGY | 1995年 / 50卷 / 01期

关键词：

CRY J I; JAPANESE CEDAR POLLEN; PECTATE LYASE; POLLINOSIS;

D O I：

10.1111/j.1398-9995.1995.tb02489.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

In the course of analyzing the partial amino acid sequences of Cry j I, a major allergen of Japanese cedar (Cryptomeria japonica) pollen, we found a peptide fragment which has a significant homology to some pectate lyase isozymes secreted by plant pathogenic bacteria. Therefore, we investigated whether Cry j I has pectate lyase activity. Cry j I reacted with polygalacturonic acid, resulting in the release of unsaturated uronide products. The optimum temperature and pH for the reaction were 60-70 degrees C and pH 10. The enzymatic reaction had an absolute Ca2+ ion requirement. These characteristics were very compatible with the character of the pectate lyase isozymes reported previously. These results clearly show that Cry j I has pectate lyase activity.

引用

页码：90 / 93

页数：4

共 21 条

[1] POLYGALACTURONIC ACID TRANS-ELIMINASE IN OSMOTIC SHOCK FLUID OF ERWINIA-RUBRIFACIENS - CHARACTERIZATION OF PURIFIED ENZYME AND ITS EFFECT ON PLANT-CELLS [J].