GAS-CHROMATOGRAPHIC ANALYSIS OF POLY(3-HYDROXYALKANOATES) IN BACTERIA

被引：49

作者：

HUIJBERTS, GNM ^{[1
]}

VANDERWAL, H ^{[1
]}

WILKINSON, C ^{[1
]}

EGGINK, G ^{[1
]}

机构：

[1] DLO,ATO,AGROTECHNOL RES INST,POB 17,6700 AA WAGENINGEN,NETHERLANDS

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 03期

关键词：

D O I：

10.1007/BF00161588

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The accuracy and reproducibility of the gas-chromatographic method for the analysis of PHB and PHA in whole cells of Alcaligenes eutrophus H16 and Pseudomonas putida KT2442 were determined. It was found that for analysis of PHA the methanolysis time in the assay had to be increased to 4 h. Accuracy of the PHB and PHA assay were 0.018 mg and 0.304 mg respectively, based on estimation of the measurement error.

引用

页码：187 / 192

页数：6

共 3 条

[1] RAPID GAS-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF POLY-BETA-HYDROXYBUTYRIC ACID IN MICROBIAL BIOMASS [J].

BRAUNEGG, G ;

SONNLEITNER, B ;

LAFFERTY, RM .

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 6 (01) :29-37

[2] PSEUDOMONAS-PUTIDA KT2442 CULTIVATED ON GLUCOSE ACCUMULATES POLY(3-HYDROXYALKANOATES) CONSISTING OF SATURATED AND UNSATURATED MONOMERS [J].

HUIJBERTS, GNM ;

EGGINK, G ;

DEWAARD, P ;

HUISMAN, GW ;

WITHOLT, B .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (02) :536-544

[3] FORMATION OF POLYESTERS BY PSEUDOMONAS-OLEOVORANS - EFFECT OF SUBSTRATES ON FORMATION AND COMPOSITION OF POLY-(R)-3-HYDROXYALKANOATES AND POLY-(R)-3-HYDROXYALKENOATES [J].

LAGEVEEN, RG ;

HUISMAN, GW ;

PREUSTING, H ;

KETELAAR, P ;

EGGINK, G ;

WITHOLT, B .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (12) :2924-2932

← 1 →