IMPROVED RAPID SLIDE AGGLUTINATION-TEST FOR PRESUMPTIVE DIAGNOSIS OF CANINE BRUCELLOSIS

被引：35

作者：

BADAKHSH, FF

CARMICHAEL, LE

DOUGLASS, JA

机构：

[1] PITMAN MOORE INC, WASHINGTON CROSSING, NJ 08560 USA

[2] CORNELL UNIV, NEW YORK STATE COLL VET MED, BAKER INST, ITHACA, NY 14853 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1982年 / 15卷 / 02期

关键词：

D O I：

10.1128/JCM.15.2.286-289.1982

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs was developed. The method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 M 2-mercaptoethanol solution. Equal volumes (0.05 ml) of the treated serum and the B. canis plate antigen were mixed. Agglutination results were read within 2 min. Clinical studies showed 100% agreement between this method and the conventional 2-mercaptoethanol tube agglutination test. Excellent correlation was shown between cultural isolation and the modified rapid slide agglutination test using sera from experimentally infected dogs.

引用

页码：286 / 289

页数：4