INDUCTION OF THE ATP-DEPENDENT PROTEOLYTIC SYSTEM IN GUINEA-PIG RETICULOCYTE LYSATES BY TRIIODOTHYRONINE

被引:3
作者
ARUMANAYAGAM, M
SWAMINATHAN, R
机构
[1] Department of Chemical Pathology The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
关键词
D O I
10.1016/0024-3205(91)90387-Q
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The mechanism involved in the decreased numbers of several trans-membrane proteins such as sodium pump sites, sodium-lithium countertransport, sodium potassium cotransport proteins, proteins mediating the passive efflux of sodium and insulin receptors in erythrocytes from patients with hyperthyroidism is not known. The ATP-dependent proteolytic system which is involved in the loss of trans-membrane proteins during the maturation of the reticulocyte may be involved in the accelerated loss of these membrane proteins. Therefore, the effect of thyroid hormones on the ATP-dependent proteolytic activity of reticulocyte lysates was examined in this study. Reticulocytosis was induced in 14 guinea pigs by phenylhydrazine hydrochloride injections for 5 consecutive days followed by 2 days of rest. T3 (10-mu-g/100g body weight) was injected into 7 animals on day 4 and day 6. Reticulocyte-rich blood was withdrawn on day 8. Oxygen consumption determined 24 hours after injection of T3 was 25% higher (p < 0.01) and T3 treated animals had a 2.5 fold higher (p < 0.01) weight loss than control animals. The ATP-dependent proteolytic activity measured in reticulocyte lysates using I-125 labelled lysozyme was 3.6 fold higher in the T3 than in the control group of guinea pigs (p < 0.01). We conclude that thyroid hormones induce the ATP-dependent proteolytic activity of reticulocyte lysates which may be responsible for the reduced number of several trans-membrane proteins found in erythrocytes from patients with hyperthyroidism.
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页码:1369 / 1376
页数:8
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