A NOVEL TRANSCRIPTIONAL ACTIVATOR ORIGINATING FROM AN UPSTREAM PROMOTER IN THE HUMAN GROWTH-HORMONE GENE

Transcription of the human growth hormone gene can start in vitro and in vivo 197 base pairs upstream from the cap site of growth hormone mRNA (Courtois, S. J., Lafontaine, D., and Rousseau, G. G. (1992) J. Biol. Chen. 267, 19736-19743). We have now characterized the mRNA that originates from this optional promoter and have found that it occurs in human hypophysis and placenta but not in 10 other tissues, This mRNA contains an open reading frame for a protein of 107 residues that shares sequence similarity with three domains of hepatic nuclear factor-1 alpha. With antibodies directed against a peptide corresponding to the C terminus of this protein, immunoreactive material was detected in a subset of cells of the adenohypophysis. When fused to the DNA-binding domain of the yeast transcription factor GALE, the protein stimulated transcription from a GAL4-sensitive reporter gene in transiently transfected pituitary and placental cells.